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 | Acesso ao texto completo restrito à biblioteca da Embrapa Arroz e Feijão. Para informações adicionais entre em contato com cnpaf.biblioteca@embrapa.br. |
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Registro Completo |
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Biblioteca(s): |
Embrapa Arroz e Feijão. |
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Data corrente: |
27/09/2023 |
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Data da última atualização: |
27/09/2023 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
CÔRTES, M. V. de C. B.; BARBOSA, E. T.; OLIVEIRA, M. I. de S.; MACIEL, L. H. R.; LOBO JUNIOR, M.; CONTESINI, F. J.; FILIPPI, M. C. C. de; SILVA-LOBO, V. L. |
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Afiliação: |
MARCIO VINICIUS DE C BARROS CORTES, CNPAF; ELDER TADEU BARBOSA, CNPAF; MAYTHSULENE INACIO DE SOUSA OLIVEIRA; LIRIEL HELEN RODRIGUES MACIEL, UNIVERSIDADE FEDERAL DE GOIÁS; MURILLO LOBO JUNIOR, CNPAF; FABIANO JARES CONTESINI, UNIVERSITY OF DENMARK; MARTA CRISTINA CORSI DE FILIPPI, CNPAF; VALACIA LEMES DA SILVA LOBO, CNPAF. |
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Título: |
Trichoderma harzianum marker-free strain construction based on efficient CRISPR/Cas9 recyclable system: A helpful tool for the study of biological control agents. |
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Ano de publicação: |
2023 |
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Fonte/Imprenta: |
Biological Control, v. 184, 105281, Sept. 2023. |
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ISSN: |
1049-9644 |
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DOI: |
https://doi.org/10.1016/j.biocontrol.2023.105281 |
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Idioma: |
Inglês |
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Conteúdo: |
This article reports the first successful marker-free genetic engineering of Trichoderma harzianum, a widely studied biocontrol agent with great potential for use in sustainable agriculture. T. harzianum has been shown to control plant diseases, promote plant growth, and improve plant tolerance to abiotic stress. A specific CRISPR/Cas9 gene-editing system, based in self-replicative AMA1-plasmid, was used to generate T. harzianum mutant strains, targeting the albA (pks4) and ku70 genes, involved in melanin synthesis and non-homologous end joining DNA repair pathway, respectively. The resulting albA-deficient strain displayed a loss of pigmentation in their conidia, confirming phenotypically and genotypically the efficacy of the CRISPR/Cas9 system in generating targeted gene knockouts in T. harzianum. A marker-free ?ku70 strain was also constructed, with the maintenance of its original fitness, providing a helpful tool for precise genetic engineering in T. harzianum, due to NHEJ-deficiency, able to be used in in vitro and in vivo (in planta) studies. The study highlights the potential of using CRISPR/Cas9 technology to facilitate the study of the molecular processes involved in the biocontrol and plant growth-promoting properties of T. harzianum. |
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Palavras-Chave: |
AMA1-plasmid; Biocontrol; Gene edition; Molecular technology; NHEJ. |
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Thesagro: |
Genética Molecular; Trichoderma Harzianum. |
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Thesaurus Nal: |
Abiotic stress; Genetic engineering; Plant growth-promoting rhizobacteria. |
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Categoria do assunto: |
X Pesquisa, Tecnologia e Engenharia |
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Marc: |
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001 2156940
005 2023-09-27
008 2023 bl uuuu u00u1 u #d
022 $a1049-9644
024 7 $ahttps://doi.org/10.1016/j.biocontrol.2023.105281$2DOI
100 1 $aCÔRTES, M. V. de C. B.
245 $aTrichoderma harzianum marker-free strain construction based on efficient CRISPR/Cas9 recyclable system$bA helpful tool for the study of biological control agents.$h[electronic resource]
260 $c2023
520 $aThis article reports the first successful marker-free genetic engineering of Trichoderma harzianum, a widely studied biocontrol agent with great potential for use in sustainable agriculture. T. harzianum has been shown to control plant diseases, promote plant growth, and improve plant tolerance to abiotic stress. A specific CRISPR/Cas9 gene-editing system, based in self-replicative AMA1-plasmid, was used to generate T. harzianum mutant strains, targeting the albA (pks4) and ku70 genes, involved in melanin synthesis and non-homologous end joining DNA repair pathway, respectively. The resulting albA-deficient strain displayed a loss of pigmentation in their conidia, confirming phenotypically and genotypically the efficacy of the CRISPR/Cas9 system in generating targeted gene knockouts in T. harzianum. A marker-free ?ku70 strain was also constructed, with the maintenance of its original fitness, providing a helpful tool for precise genetic engineering in T. harzianum, due to NHEJ-deficiency, able to be used in in vitro and in vivo (in planta) studies. The study highlights the potential of using CRISPR/Cas9 technology to facilitate the study of the molecular processes involved in the biocontrol and plant growth-promoting properties of T. harzianum.
650 $aAbiotic stress
650 $aGenetic engineering
650 $aPlant growth-promoting rhizobacteria
650 $aGenética Molecular
650 $aTrichoderma Harzianum
653 $aAMA1-plasmid
653 $aBiocontrol
653 $aGene edition
653 $aMolecular technology
653 $aNHEJ
700 1 $aBARBOSA, E. T.
700 1 $aOLIVEIRA, M. I. de S.
700 1 $aMACIEL, L. H. R.
700 1 $aLOBO JUNIOR, M.
700 1 $aCONTESINI, F. J.
700 1 $aFILIPPI, M. C. C. de
700 1 $aSILVA-LOBO, V. L.
773 $tBiological Control$gv. 184, 105281, Sept. 2023.
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Embrapa Arroz e Feijão (CNPAF) |
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| Registros recuperados : 16 | |
| 2. |  | ROMBKE, J.; HANAGARTH, W.; HOEFER, H.; MARTIUS, C. Bait-lamina. In: SHIFT PROJECT ENV 52 (Manaus-AB). Soil fauna and litter decomposition in primary and secondary forests and a mixed culture system in Amazonia. Manaus: SMNK/Embrapa Amazonia Ocidental, 1999. p. 256-264. Final Report 1996-1999.| Biblioteca(s): Embrapa Amazônia Ocidental. |
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| 8. | | HÖFFER, H.; HANAGARTH, W.; GARCIA, M. V. B.; MARTIUS, C.; FRANKLIN, E.; RÖMBKE, J.; BECK, L. Structure and function of soil fauna communities in Amazonian anthropogenic and natural ecosystems. European Journal of Soil Biology, v. 37, n. 4, p. 229-235, nov./dez. 2001.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: -- - -- |
| Biblioteca(s): Embrapa Amazônia Ocidental. |
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| 11. | | GARCIA, M.; BECK, L.; FÖRSTER, B.; FRANKLIN, E.; HANAGARTH, W.; HÖFER, H.; LUIZÃO, F.; LUIZÃO, R.; MARTIUS, C.; MORAIS, J. W.; RÖMBKE, J. Abundance, biomass and diversity of the soil fauna in degraded areas under recuperation in the Central Amazon Region. In: GERMAN-BRAZILIAN WORKSHOP ON NEOTROPICAL ECOSYSTEMS. ACHIEVEMENTS AND PROSPECTS OF COOPERATIVE RESEARCH, 2000, Hamburg. Proceedings... Hamburg: SHIFT: MADAM: WAVES, 2002. p. 126.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Amazônia Ocidental. |
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| 12. | | GARCIA, M.; BECK, L.; FORSTER, B.; FRANKLIN, E.; HANAGARTH, W.; HOFER, H.; LUIZAO, F.; LUIZAO, R.; MARTIUS, C.; MORAIS, J. W.; ROMBKE, J. Abundance, biomass and diversity of the soil fauna in degrated areas under recuperation in the central Amazon region. In: GERMAN-BRAZILIAN WORKSHOP ON NEOTROPICAL ECOSYSTEMS, 2000, Hamburg. Achievements and prospects of cooperative research: program and abstracts. Hamburg: University of Hamburg / Institute of Chemistry, 2000. p. 27.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Amazônia Ocidental. |
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| 13. | | HOFER, H.; BECK, L.; FORSTER, B.; GARCIA, M.; HANAGARTH, W.; LUIZAO, F.; LUIZAO, R.; MARTIUS, C.; MORAIS, J. W.; ROMBKE, J. The function of the soil macrofauna in decomposition processes in central Amazonian polyculture systems and forests. In: GERMAN-BRAZILIAN WORKSHOP ON NEOTROPICAL ECOSYSTEMS, 2000, Hamburg. Program and abstracts... Hamburg: University, 2000. p. 26.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Amazônia Ocidental. |
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| 14. | | HÖFER, H.; BECK, L.; FÖRSTER, B.; GARCIA, M.; HANAGARTH, W.; LUIZÃO, F.; LUIZÃO, R.; MARTIUS, C.; MORAIS, J. W.; RÖMBKE, J. The function of the soil macrofauna in decomposition processes in Central Amazonian polyculture systems and forests. In: GERMAN-BRAZILIAN WORKSHOP ON NEOTROPICAL ECOSYSTEMS. ACHIEVEMENTS AND PROSPECTS OF COOPERATIVE RESEARCH, 2000, Hamburg. Proceedings... Hamburg: SHIFT: MADAM: WAVES, 2002. p. 127.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Amazônia Ocidental. |
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| 15. | | RODRIGUES, M. do R.; BECK, L.; DIAS, M. C.; FÖRSTER, B.; GARCIA, T.; GARCIA, M.; HANAGARTH, W.; HÖFER, H.; MARTIUS, C.; RÖMBKE, J.; XAVIER, J. J. Management of leguminous cover crops to improve soil fertility in Central Amazonia. In: GERMAN-BRAZILIAN WORKSHOP ON NEOTROPICAL ECOSYSTEMS. ACHIEVEMENTS AND PROSPECTS OF COOPERATIVE RESEARCH, 2000, Hamburg. Proceedings... Hamburg: SHIFT: MADAM: WAVES, 2002. p. 188.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Amazônia Ocidental. |
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| 16. | | HÖFER, H.; BURGER, D.; FÖRSTER, B.; GARCIA, M. V. B.; GARCIA, T.; HANAGARTH, W.; MARTIUS, C.; RODRIGUES, M. R. L.; RÖMBKE, J.; SCHMIDT, P.; VERHAAGH, M. Managing plant residues to improve soil fertility in central Amazonian agroecosystems. An integrated approach with agricultural trials, controlled field and laboratory experiments, ecotoxicological tests and a screenning of eight different plantations with regard to soil macrofauna and decomposition. In: INTERNATIONAL COLLOQUIUM ON SOIL ZOOLOGY AND ECOLOGY, 14., 2004, Mont Saint Aignan. Abstracts... Mont Saint Aignan: Université de Rouen, 2004. p. 98.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Amazônia Ocidental. |
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| Registros recuperados : 16 | |
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| Nenhum registro encontrado para a expressão de busca informada. |
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